Translational_Unit

Part:BBa_K4158007:Design

Designed by: Izumi Kim   Group: iGEM22_Waseda_Tokyo   (2022-09-18)


RBS-AtzC


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal EcoRI site found at 1133
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal EcoRI site found at 1133
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal EcoRI site found at 1133
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal EcoRI site found at 1133
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal EcoRI site found at 1133
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

If you want to get same plasmid that we experimented with, construct the plasmid along this way below.

1. Prepare pJL1 cloning vector (addgene Plasmid #69496)and this part as BioBrick.

2. Restriction and insertion cloning with pJL1 and this BioBrick productions and restriction enzymes XbaI and salI.

Source

test

References

[1] Liu, X., Silverman, A. D., Alam, K. K., Iverson, E., Lucks, J. B., Jewett, M. C., and Raman, S. (2020) Design of a Transcriptional Biosensor for the Portable, On-Demand Detection of Cyanuric Acid. ACS Synth. Biol. 9 (1), 84– 94